YouTube TV subscribers will have to look elsewhere for Disney's content as of Saturday.
The sides were unable to reach a new agreement that would have kept several Disney networks -- including ESPN, the SEC Network, ABC, the Disney Channel, FX and National Geographic -- on the streaming app.
All of those channels were gone from YouTube TV at midnight on Saturday.
The move comes as little surprise, with the sides previously reported to be far apart on a new deal.
In response, YouTube TV cut its monthly subscription rate by $15 to account for the loss of the channels.
Both companies played the blame game on Friday night, with Disney claiming that YouTube TV had let down its customers by declining to reach a "fair" deal.
However, both parties expressed interest in continuing negotiations and getting a deal done.
YouTube TV touted months of "good faith" bargaining and said it still hopes to restore the lost channels for its subscribers.
The move comes after talks have escalated into public campaigning recently, with ESPN running commercials warning YouTube TV subscribers that the Disney content could soon be gone.
Earlier this year, a similar carriage dispute unfolded between YouTube TV and NBC Universal, but a last-minute deal was reached that helped avert the loss of any content.
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